The hook-like extension (amino acids 59вЂ“87) embraces pUL50 and contributes 1510 в„« 2 to the total interface area (1880 в„« 2). pUL53-specific features include a zinc-binding site and a hook-like N-terminal extension, the latter representing a hallmark element of the pUL50-pUL53 interaction. Both proteins adopt a globular fold with mixed О± and ОІ secondary structure elements. Here, we report the crystal structure of the pUL50-pUL53 heterodimer (amino acids 1вЂ“175 and 50вЂ“292, respectively) at 2.44 в„« resolution. pUL50 and pUL53 heterodimerize and form a core nuclear egress complex (NEC), which is anchored to the inner nuclear membrane and provides a scaffold for the assembly of a multimeric viral-cellular NEC. Thus, the role of two essential and conserved viral nuclear egress proteins, pUL50 and pUL53, is pivotal. Nuclear replication of cytomegalovirus relies on elaborate mechanisms of nucleocytoplasmic egress of viral particles.

Glycobiology and Extracellular Matrices.

150000001413 amino acids Chemical group 0.000 claims description 68.

108090000623 proteins and genes Proteins 0.000 claims description 141.

102000004169 proteins and genes Human genes 0.000 claims description 141.

Assignors: UNIVERSITY OF LEEDS Status Active legal-status Critical Current Anticipated expiration legal-status Critical Links Assignors: MEDICAL RESEARCH COUNCIL Assigned to APTUSCAN LIMITED reassignment APTUSCAN LIMITED ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: APTUSCAN LIMITED Assigned to UNIVERSITY OF LEEDS reassignment UNIVERSITY OF LEEDS ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: GENDRA, ELISENDA Assigned to AVACTA LIFE SCIENCES LIMITED reassignment AVACTA LIFE SCIENCES LIMITED ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: FERRIGNO, PAUL KO Assigned to UNIVERSITY OF LEEDS reassignment UNIVERSITY OF LEEDS ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Priority to GB0807065.8 priority Critical Priority to GBGB0807065.8A priority patent/GB0807065D0/en Priority to PCT/GB2009/050380 priority patent/WO2009136182A1/en Priority to US98810610A priority Application filed by AVACTA LIFE SCIENCES Ltd filed Critical AVACTA LIFE SCIENCES Ltd Priority to US14/478,910 priority patent/US9447170B2/en Publication of US20160024184A1 publication Critical patent/US20160024184A1/en Publication of US9447170B2 publication Critical patent/US9447170B2/en Application granted granted Critical Assigned to UNIVERSITY OF LEEDS, MEDICAL RESEARCH COUNCIL reassignment UNIVERSITY OF LEEDS ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Original Assignee AVACTA LIFE SCIENCES Ltd Priority date (The priority date is an assumption and is not a legal conclusion.

Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.) ( en Inventor Paul Ko Ferrigno Elisenda Gendra Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Granted Application number US14/478,910 Other versions US9447170B2 Google Patents US20160024184A1 - Modified stefin a scaffold proteins US20160024184A1 - Modified stefin a scaffold proteins

Secondary structure protein scaffold